Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180759_c4wq ACTCTCAACCCAAAAAGTCTCTTTATTGATTG(+AAAAAA)GTACCATACATGACTCAAATGGCCAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606180756_sihm CTGGGTGCCCCATCTTCCTGATTTCTCCCTTG(+AAAAAA)GATCCTGGGGTTTATACCTACAGCCCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240606180754_60mp TAGCCCCTAAGGGCTGTAGGTATAAACCCCAG(+AAAAAA)GATCCAAGGGAGAAATCAGGAAGATGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606180753_d7si AGAAATTCACCATACACTTCTCAGTGTCTTAG(+AAAAAA)GATCCAGATGTCCGGTCCATCCCTGAAACCT NGG 10 8 17 8 24 0 100 5 10
    240606180751_jkl1 GGGAGAGGTTTCAGGGATGGACCGGACATCTG(+AAAAAA)GATCCTAAGACACTGAGAAGTGTATGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606180750_pukk GTCTGTTCTCCTGAAATCCCAGGATTGGCAGG(+AAAAAA)GTACCTGGCCAGGCCAACCAGGGCCACTCTG NGG 10 8 17 8 24 0 100 5 10
    240606180748_10iv GGGTACAGAGTGGCCCTGGTTGGCCTGGCCAG(+AAAAAA)GTACCCTGCCAATCCTGGGATTTCAGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606180746_zr5l GCCAACATCTTGGAGCTGGGCTCGTGCCTGAG(+AAAAAA)GTACCAGTCTGCTTTGCTTTGAAACGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606180745_nh30 GTCCTTCCTTCGTTTCAAAGCAAAGCAGACTG(+AAAAAA)GTACCTCAGGCACGAGCCCAGCTCCAAGATG NGG 10 8 17 8 24 0 100 5 10
    240606180743_ken0 GCCATCACCAAAGGGTGCAGCCACAGGGCTGG(+AAAAAA)GTACCTGCATGCTTCATCTCCACCTGAAGGC NGG 10 8 17 8 24 0 100 5 10