Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175026_ebkl ATGTCATTAAACGGAAAGACTGTGCTGGGCAG(+AAAAAA)GATCCTGCCATCTCTAGTACAGTTATGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606175024_qjtj AGGAGACTAATGCCCTCTGATGATCTCTATGG(+AAAAAA)GTACCAGGCACACATAGACATACAGGTATGC NGG 10 8 17 8 24 0 100 5 10
    240606175022_brec TACCTGCATACCTGTATGTCTATGTGTGCCTG(+AAAAAA)GTACCCATAGAGATCATCAGAGGGCATTAGT NGG 10 8 17 8 24 0 100 5 10
    240606175021_i3nd AGTGGTTCAGAGAACTGGTTGTTCTTGCAGAG(+AAAAAA)GATCCTAGGTTCAGTTCCTAGTACCTGCATG NGG 10 8 17 8 24 0 100 5 10
    240606175019_a4vu TCTGCCATGCAGGTACTAGGAACTGAACCTAG(+AAAAAA)GATCCTCTGCAAGAACAACCAGTTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606175017_6ehi ACCCTCATACTGTCAGATACGTTTTGATAAGG(+AAAAAA)GATCCAAGAGCAGTAAGGGTCAGTCTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606175016_lj6g CTTTGTTGAAAGACTGACCCTTACTGCTCTTG(+AAAAAA)GATCCCTTATCAAAACGTATCTGACAGTATG NGG 10 8 17 8 24 0 100 5 10
    240606175015_idh4 CCAGGCATGTGCGTTAAGGGAGCTCTCAAACG(+AAAAAA)GATCCCTCCAGCTGCATCCTGCCTTTCACCA NGG 10 8 17 8 24 0 100 5 10
    240606175013_xik6 TTCTCTGGTGAAAGGCAGGATGCAGCTGGAGG(+AAAAAA)GATCCGTTTGAGAGCTCCCTTAACGCACATG NGG 10 8 17 8 24 0 100 5 10
    240606175011_i8gt TCTGCTGTCTGCTGAGGGAGAGGGGACCTGTG(+AAAAAA)GATCCTGACAACAGGCCACGCAGAGGTGGGA NGG 10 8 17 8 24 0 100 5 10