Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175122_mk4a CAGGTGTCCAAATAGGCTAGAGAAAAGTATAG(+AAAAAA)GATCCCCTGGATCTGGAGTTATAGACAGTAG NGG 10 8 17 8 24 0 100 5 10
    240606175121_vzk0 GCCTGTCATGATGGTGCTGGGAAACAAACTTG(+AAAAAA)GATCCTCTGAAAGAGCAGTGAGTACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606175119_oew2 AGTGGTTAAAAGTACTCACTGCTCTTTCAGAG(+AAAAAA)GATCCAAGTTTGTTTCCCAGCACCATCATGA NGG 10 8 17 8 24 0 100 5 10
    240606175118_8wqt CTGGTGCTCAAGAAAGCTAGCAGAGGGCGCTG(+AAAAAA)GATCCCATGGAACTGGAGTTAGGGAGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606175116_ps0v GCTTATCACCCTCCCTAACTCCAGTTCCATGG(+AAAAAA)GATCCAGCGCCCTCTGCTAGCTTTCTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606175114_xw2f CCTCTAAGTGCCAAACAGACCATGCCCGCTGG(+AAAAAA)GATCCGGATCAGAAGAAAGCAGGATCGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606175112_uxqa GGGCTCACTCGATCCTGCTTTCTTCTGATCCG(+AAAAAA)GATCCCAGCGGGCATGGTCTGTTTGGCACTT NGG 10 8 17 8 24 0 100 5 10
    240606175111_8zmy ATAACACTGCCAATGGTTAAGTCACAGTCCTG(+AAAAAA)GATCCTTAGCTATTACCATCCCTCAGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606175110_5mqd TGGATGCTTTCTGAGGGATGGTAATAGCTAAG(+AAAAAA)GATCCAGGACTGTGACTTAACCATTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606175108_85s1 CTGGGGTGCAGTGTGAGGGTGGGCGCTTGTAG(+AAAAAA)GATCCCAAACTGGTGGGGGTGAGAAAGGGCA NGG 10 8 17 8 24 0 100 5 10