Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175152_ke1n TCATCTGGGTGTGGGAGGCCAGTCTGAGCGGG(+AAAAAA)GATCCCCCCCCAGGGCACAGCGCAACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606175151_d47r GCCCTGGATGGATGACTCTGGAATCCAGTATG(+AAAAAA)GTACCATGGAAGGAGCACTGGACTGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606175149_4x64 TCCAGGGCTCTCAGTCCAGTGCTCCTTCCATG(+AAAAAA)GTACCATACTGGATTCCAGAGTCATCCATCC NGG 10 8 17 8 24 0 100 5 10
    240606175147_k2nm AGGAAAGTATGGTTTCCCCAGGTCTGAACTGG(+AAAAAA)GTACCCCCAGGGTGTTTACTAACTCTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606175146_jjl5 ACCCTTCACCAGAGTTAGTAAACACCCTGGGG(+AAAAAA)GTACCCAGTTCAGACCTGGGGAAACCATACT NGG 10 8 17 8 24 0 100 5 10
    240606175144_oolp GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+AAAAAA)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606175143_g5x9 TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+AAAAAA)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606175142_qixo GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+AAAAAA)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606175141_s0oc TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+AAAAAA)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606175140_eaam GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+AAAAAA)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10