Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175251_t38r TGAAGTATAGTGCAAGAAAGAAATCTAAAATG(+AAAAAA)GTACCAGTACTGCTGTGAGTTCATCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606175249_cbcz TCTGGGGATGTGGGATCTGCTTCTAAGTGTGG(+AAAAAA)GTACCATGTCTACTCTCACTTTATAAAATTA NGG 10 8 17 8 24 0 100 5 10
    240606175248_8txf AAGGTTAATTTTATAAAGTGAGAGTAGACATG(+AAAAAA)GTACCCACACTTAGAAGCAGATCCCACATCC NGG 10 8 17 8 24 0 100 5 10
    240606175246_grgm CCTGTAGCAGGCTCTGCTTTTCGGGTCCCATG(+AAAAAA)GTACCAGATTCTTGAAATAAGGTCATGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606175244_hkzq CTAACTCTTCATGACCTTATTTCAAGAATCTG(+AAAAAA)GTACCATGGGACCCGAAAAGCAGAGCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606175243_5rvr GCTGATGATATGTACGTGCCTGAAACTGGATG(+AAAAAA)GTACCTTTAACTGACACTTAGAAAGCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606175242_dxjt GCTAATAAAAGCTTTCTAAGTGTCAGTTAAAG(+AAAAAA)GTACCATCCAGTTTCAGGCACGTACATATCA NGG 10 8 17 8 24 0 100 5 10
    240606175241_e8sj CAGTGCACTGCACCTCGGAACACATGCAGTTG(+AAAAAA)GATCCTAAGGATGTTTAAAAGCGATTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606175239_y218 GAGACTTTAAAATCGCTTTTAAACATCCTTAG(+AAAAAA)GATCCAACTGCATGTGTTCCGAGGTGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606175237_1aol ACAGCCGCATTCCAGGGTGGGAGAGCGTGCTG(+AAAAAA)GTACCATCTTCTCTCAAGTAGCTCTGTCATC NGG 10 8 17 8 24 0 100 5 10