Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175337_orty TAGCTTCGTCCTCATTCCCTATGCGCACCGAG(+AAAAAA)GTACCCGCCCCATAGAAAGCTCTAGAACCTG NGG 10 8 17 8 24 0 100 5 10
    240606175335_5ujl TCATGTTTCTCACTGGGGTCTTGGAACCTGTG(+AAAAAA)GATCCCAGTCCCTATGTGATATGCTCAAATG NGG 10 8 17 8 24 0 100 5 10
    240606175332_kj1x CAGGGCATTTGAGCATATCACATAGGGACTGG(+AAAAAA)GATCCACAGGTTCCAAGACCCCAGTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606175331_i31d CATTCTACCCCATTATTTTAGTATTGGTATTG(+AAAAAA)GATCCAATCAGGGCAAGAACATTGTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240606175330_l9nu TTTAATAACATACAATGTTCTTGCCCTGATTG(+AAAAAA)GATCCAATACCAATACTAAAATAATGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240606175329_7qo5 GCTTCCCTCAACCTCTCCCTGGCCTGGAGGAG(+AAAAAA)GATCCCAGAGGAAAGGGTTTTCTATGACTGA NGG 10 8 17 8 24 0 100 5 10
    240606175327_tjan CTAGCTCAGTCATAGAAAACCCTTTCCTCTGG(+AAAAAA)GATCCTCCTCCAGGCCAGGGAGAGGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606175326_owq6 ACACGTGCTGTTATCTAACAAGGAAAAGTTTG(+AAAAAA)GATCCTGAGAAAGCAAATAAACATTTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606175324_61l5 AAAATACTTGAAATGTTTATTTGCTTTCTCAG(+AAAAAA)GATCCAAACTTTTCCTTGTTAGATAACAGCA NGG 10 8 17 8 24 0 100 5 10
    240606175323_6te0 ACAGGAAGTTCCTTAAAAGCATATCATCTTAG(+AAAAAA)GTACCCTCTCTGAGGGTATGACTTCAAATTA NGG 10 8 17 8 24 0 100 5 10