Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180458_4no1 GTTTCTTTCCGAGCTGATGACAAGACTGAATG(+AAAAAA)GTACCTTTTCTTTCTGATCGGTTCTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240606180456_k2c3 GGCCAATGTTTAGAACCGATCAGAAAGAAAAG(+AAAAAA)GTACCATTCAGTCTTGTCATCAGCTCGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606180455_7zz6 CCCCAAGACTTTGTTTTATCGGAGAGGAAAAG(+AAAAAA)GTACCCCATTTCATTCATTGGTGTAAGGATG NGG 10 8 17 8 24 0 100 5 10
    240606180452_urs8 GAAAACATCCTTACACCAATGAATGAAATGGG(+AAAAAA)GTACCTTTTCCTCTCCGATAAAACAAAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606180450_pn5n AACTTAAATAAAAATAGCACCAGAAGGACCAG(+AAAAAA)GTACCAATAACATATTTCACCCATCCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240606180449_oo3p TAAAATTACTGGATGGGTGAAATATGTTATTG(+AAAAAA)GTACCTGGTCCTTCTGGTGCTATTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240606180447_ycme TACAAACTAATTTTTAGTAGCTGATAATACAG(+AAAAAA)GATCCCACAGTCTATTTACATAAAAGCCCGT NGG 10 8 17 8 24 0 100 5 10
    240606180446_y3ye TAAAAACGGGCTTTTATGTAAATAGACTGTGG(+AAAAAA)GATCCTGTATTATCAGCTACTAAAAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240606180444_qbpn TCTCTGGAGAGACCCTGGCCCCAGGACAACAG(+AAAAAA)GTACCAGGTTTCCCAGCTATCTCATTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606180442_xddq CTGGCAGAGAAATGAGATAGCTGGGAAACCTG(+AAAAAA)GTACCTGTTGTCCTGGGGCCAGGGTCTCTCC NGG 10 8 17 8 24 0 100 5 10