Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175510_chlh TGATAAAAAATAAAAATAAAATGTGAACTTGG(+AAAAAA)GTACCATCAGAAACGATGTTAAAGGTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606175508_yaga ATGTCTTATTCTAACCCTATGATCTTCGCTGG(+AAAAAA)GATCCAGAGGGCCACGTGGCTCTGTTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606175506_q7dx TGCTGTGCTCAACAGAGCCACGTGGCCCTCTG(+AAAAAA)GATCCCAGCGAAGATCATAGGGTTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606175504_va4j GTGAAGAATAATCTATCTCTATTCCTGCCAGG(+AAAAAA)GTACCTTATGTACTAAAGCGCCTTACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606175503_xnop TAATTCCTGAGTAAGGCGCTTTAGTACATAAG(+AAAAAA)GTACCCTGGCAGGAATAGAGATAGATTATTC NGG 10 8 17 8 24 0 100 5 10
    240606175501_544k CATAGACCTCTCCTCTGCCACTGTATCTCGGG(+AAAAAA)GTACCATTCAAGCACAGCCTGAGAGCATAAG NGG 10 8 17 8 24 0 100 5 10
    240606175459_a7pu GTGACCTTATGCTCTCAGGCTGTGCTTGAATG(+AAAAAA)GTACCCCGAGATACAGTGGCAGAGGAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606175458_4x26 TGCAGGAATCCTGCTACTCAGCCCTGCACTGG(+AAAAAA)GTACCACCCCCAAGCCCCCATTCCTTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606175456_bllh ATGCCAGGGGAAGGAATGGGGGCTTGGGGGTG(+AAAAAA)GTACCCAGTGCAGGGCTGAGTAGCAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240606175455_nvst ACTCATGTGACTGGTTTATTACAGTATGTAAG(+AAAAAA)GATCCAAAGAAACTGATTCGTATCCACGCAG NGG 10 8 17 8 24 0 100 5 10