Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175612_ccvm CAATGGCTATCTCTGAGGGTCTAATCAAGCAG(+AAAAAA)GATCCTGGAGGAAGCTCAAGTTGCTCGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606175611_lco0 TATCCCGAGGACAGTTACTAAGCTATACTTGG(+AAAAAA)GATCCCACTCAGAAGGTTTCAGAACTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606175609_gh2k GAAGGACCCCAGTTCTGAAACCTTCTGAGTGG(+AAAAAA)GATCCCAAGTATAGCTTAGTAACTGTCCTCG NGG 10 8 17 8 24 0 100 5 10
    240606175607_t1ig CCATCCACACCCTGCAGCAAGTCCCAACCTGG(+AAAAAA)GTACCACTGTTATTATTACAAGGAAAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606175606_y6ne TTTAACTTTCTTTCCTTGTAATAATAACAGTG(+AAAAAA)GTACCCAGGTTGGGACTTGCTGCAGGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606175605_n25x AGAGAGGGATGTGGTGTGGACTCTGAGCTGAG(+AAAAAA)GATCCTCTCCACGTTGTGATGGGGTAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606175603_0iyy CGGCCCCTCCTACCCCATCACAACGTGGAGAG(+AAAAAA)GATCCTCAGCTCAGAGTCCACACCACATCCC NGG 10 8 17 8 24 0 100 5 10
    240606175602_3zag GGGAATCTGACACCTTCCGCTGGGTTCTGAGG(+AAAAAA)GTACCTGTACTCATGTGTACCCTGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606175601_qtc1 GTGGGGAGGGGGCAGGGTACACATGAGTACAG(+AAAAAA)GTACCCTCAGAACCCAGCGGAAGGTGTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606175559_8ck8 TGAAAACACAAGGACCTGAGCTGGGATCCTTG(+AAAAAA)GTACCCAGAGAAAAAAATCTGGGTCTGGTGG NGG 10 8 17 8 24 0 100 5 10