Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175627_w2ch CCCTGCAGCCCGACATTGCTGTAGAGCTGGAG(+AAAAAA)GATCCGTGAGATGAGGGCTACAGAGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606175626_y7nu TGCAGACCTCCGCAGCCAGTCCTCAGCGATTG(+AAAAAA)GTACCTACCTGGTCCCGGATAAATAAACGAT NGG 10 8 17 8 24 0 100 5 10
    240606175625_nz5r ACAAAATCGTTTATTTATCCGGGACCAGGTAG(+AAAAAA)GTACCAATCGCTGAGGACTGGCTGCGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606175623_c6nw AGCAGTTTAGAAGCCATGAGTCTGGGTGCCTG(+AAAAAA)GATCCTCACCGAGGCCCGGGGAGTATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606175622_241m GGAGAGCAAGATACTCCCCGGGCCTCGGTGAG(+AAAAAA)GATCCAGGCACCCAGACTCATGGCTTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240606175620_q9d8 AAATAAAATAAATAGAATAAAGTATTCAAAGG(+AAAAAA)GTACCACCACTCCAATTGCATCAAGCCACCT NGG 10 8 17 8 24 0 100 5 10
    240606175618_xawh GTCCAAGGTGGCTTGATGCAATTGGAGTGGTG(+AAAAAA)GTACCCTTTGAATACTTTATTCTATTTATTT NGG 10 8 17 8 24 0 100 5 10
    240606175617_uxg9 AAGTATTGTTTCCTAATCAGACTTTTACATTG(+AAAAAA)GTACCATGAGTTCTTTATGCTTTGCCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606175616_nzhx ACAATGTCTTGGCAAAGCATAAAGAACTCATG(+AAAAAA)GTACCAATGTAAAAGTCTGATTAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606175614_efuf ACAACCTTGCGAGCAACTTGAGCTTCCTCCAG(+AAAAAA)GATCCTGCTTGATTAGACCCTCAGAGATAGC NGG 10 8 17 8 24 0 100 5 10