Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175732_mwrj ATTTGAGGATTCCCTTGCAAGAAGCCCATGGG(+AAAAAA)GTACCATATCTCAGCCAGAGGCTTTATTCCC NGG 10 8 17 8 24 0 100 5 10
    240606175729_fab1 TTGCCTTCCAGAAAGCCTCCATCTAGCCCCAG(+AAAAAA)GTACCCCCAGGACTCCAGCCTTTAAAGAACT NGG 10 8 17 8 24 0 100 5 10
    240606175727_753e TTGTTAGTTCTTTAAAGGCTGGAGTCCTGGGG(+AAAAAA)GTACCTGGGGCTAGATGGAGGCTTTCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606175725_k9xe GACCTCCGGGGCTTTGGCCTCCTGGGACCACG(+AAAAAA)GTACCCAGGGCTCTCTGCCTTTAGGCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606175723_6rqq ACAAGAAGCTGCCTAAAGGCAGAGAGCCCTGG(+AAAAAA)GTACCGTGGTCCCAGGAGGCCAAAGCCCCGG NGG 10 8 17 8 24 0 100 5 10
    240606175722_79qs CTGTATATGTAGGTGCCCATGGAGGGATGTTG(+AAAAAA)GATCCCCTGGAGCTAGAGTTTCATGAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606175719_wjud CAACTTACACTCATGAAACTCTAGCTCCAGGG(+AAAAAA)GATCCAACATCCCTCCATGGGCACCTACATA NGG 10 8 17 8 24 0 100 5 10
    240606175716_k8pg GCCTAGTGCTACAGAGCCAGGCAGGAACCAGG(+AAAAAA)GTACCAGCCTCTAGGCCTGGTGCCTGCATCA NGG 10 8 17 8 24 0 100 5 10
    240606175715_wdu7 AGAAGTGATGCAGGCACCAGGCCTAGAGGCTG(+AAAAAA)GTACCCTGGTTCCTGCCTGGCTCTGTAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606175713_4u0o ATGAAGTTGTGAGCAGCTGCTCTGTCTTTTGG(+AAAAAA)GATCCTGTTACGATCTGACAAATTAAGTCTG NGG 10 8 17 8 24 0 100 5 10