Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175802_olsv CTGCCACCTCTGGCCCTGGAAGGGTGTGGTGG(+AAAAAA)GATCCAGGCAAGGAGAGTCCAGACGTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606175800_bevk TGGTTGACATACATGGGAATACCAAGTTTGGG(+AAAAAA)GATCCTTTTATTTAGAATATCAAGAAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606175759_rj7r GAGATTCTTTTTCTTGATATTCTAAATAAAAG(+AAAAAA)GATCCCCAAACTTGGTATTCCCATGTATGTC NGG 10 8 17 8 24 0 100 5 10
    240606175758_q2z6 TCACTGTGCTGTCCATTACTAGCTCCCAAATG(+AAAAAA)GTACCCTTCACCTTAACCATCACAGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606175756_ik4x CTACTACACTGCTGTGATGGTTAAGGTGAAGG(+AAAAAA)GTACCATTTGGGAGCTAGTAATGGACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606175755_uebp GATCAGTTCTGTTAGGGTGAGACCCGAATTTG(+AAAAAA)GATCCCCAACAGTTGTGGAGTATTTCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606175753_c1d3 CTGCCAGGATGAAATACTCCACAACTGTTGGG(+AAAAAA)GATCCAAATTCGGGTCTCACCCTAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606175752_2uog GGGTCAAACCTGCATGTGGTAATTTGATCTGG(+AAAAAA)GATCCAGATATTCAGACTTGGTTAGGTGTAC NGG 10 8 17 8 24 0 100 5 10
    240606175750_6h7h AAGGAGTACACCTAACCAAGTCTGAATATCTG(+AAAAAA)GATCCCAGATCAAATTACCACATGCAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606175749_36ez AGGAGCCCTTCTGTGTTCCTGATATTGCCATG(+AAAAAA)GATCCTTAATCTTGTCATCTTTCATATTAAG NGG 10 8 17 8 24 0 100 5 10