Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175910_zuyk GCTGTCTTCAGACACACCAGAATAGGGCATCG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606175908_k7ko GTCACATGGTGGCTCACAACCATCTGTAATGG(+AAAAAA)GATCCGATGCCCTATTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606175907_uwzp GCTGTCTTCAGACACACCAGAATAGGGCATCG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606175905_xbix GACCTAGACTTCTCTCCCTGATCCGTGGCCTG(+AAAAAA)GATCCCAAACTGAGGCAGCAGCCTCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240606175903_77yq GGCCTGGCTAGGAGGCTGCTGCCTCAGTTTGG(+AAAAAA)GATCCAGGCCACGGATCAGGGAGAGAAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606175902_bra0 CCCAGGTCCGCTCTGAACACGTGGACCAAAGG(+AAAAAA)GATCCAGGCAGCTGTGTACATTAAGATGGGT NGG 10 8 17 8 24 0 100 5 10
    240606175900_piz5 TCCCCACCCATCTTAATGTACACAGCTGCCTG(+AAAAAA)GATCCCTTTGGTCCACGTGTTCAGAGCGGAC NGG 10 8 17 8 24 0 100 5 10
    240606175859_7tuo CTGGGGTGAAGGGATAGCCCTCCCCCTGCTGG(+AAAAAA)GATCCCCCCAGCCCCTCCGGTCTGGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606175856_783z CCCCTTTCCTGCCAGACCGGAGGGGCTGGGGG(+AAAAAA)GATCCCAGCAGGGGGAGGGCTATCCCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606175854_yylw CTCCTGTTCCAAATGGTCTCCTTTAGCCTCTG(+AAAAAA)GATCCAACATGACAACCCAGACTAGAGCTTC NGG 10 8 17 8 24 0 100 5 10