Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175836_mhz7 TCTGGAAACTGTGCTGTGGCTGGGGTATGAGG(+AAAAAA)GATCCCCAGAGAACACTACAAGAAGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240606175835_6wv2 TACCCCTCCTGTGTGTGAAGGCTCTGCTGAAG(+AAAAAA)GTACCTTTGGGTCTGGTCTGACCTTACTGCT NGG 10 8 17 8 24 0 100 5 10
    240606175833_0itg GACAAAGCAGTAAGGTCAGACCAGACCCAAAG(+AAAAAA)GTACCTTCAGCAGAGCCTTCACACACAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606175831_ym8x CGGGCCACCACCTGCCTCTCTCTTATCCAGTG(+AAAAAA)GTACCGCCCAGGCCTTAAATCACCCCGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606175829_gm26 CACTGGGAACGGGGTGATTTAAGGCCTGGGCG(+AAAAAA)GTACCACTGGATAAGAGAGAGGCAGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606175828_xz0u GGTCACAGGGACAGCTCCGCTGCTGCCTCTGG(+AAAAAA)GTACCTTGAAAAGATGCCCACCACGCACCTT NGG 10 8 17 8 24 0 100 5 10
    240606175826_doef AAAGAAAGGTGCGTGGTGGGCATCTTTTCAAG(+AAAAAA)GTACCCAGAGGCAGCAGCGGAGCTGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606175823_7p9q CTGCTGCCCCTGGGTGCAGGCAGTAGCCAGGG(+AAAAAA)GATCCAGGGCCTCAGATCCAGGGTCCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606175821_2vf5 GTCCGATGCAGGACCCTGGATCTGAGGCCCTG(+AAAAAA)GATCCCCTGGCTACTGCCTGCACCCAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606175819_cins CTGCCGGGGAGATGGGCAGGGTGCACTCTGAG(+AAAAAA)GTACCCGCTCCTACAGTCCCAGAACACCCCT NGG 10 8 17 8 24 0 100 5 10