Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180014_uuq1 TAGTTTCTTTAGTAGGTCACACACAGTGGTTG(+AAAAAA)GTACCTGGAATATGTGAATGAGACAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240606180012_gqs2 GCTGGGAGGGCCGTGTACAGCCCTTGGTCTGG(+AAAAAA)GATCCATGTGTTGGTTGAGAAATCATTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606180011_xlz3 ACACTCTGGAATGATTTCTCAACCAACACATG(+AAAAAA)GATCCCAGACCAAGGGCTGTACACGGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606180009_yo6t TGTCCCCTCCCTGGTCTTATGCCACTTGCCAG(+AAAAAA)GTACCCTCAGAGCTGTGGGGTGCCTTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606180008_nucs ACGCAGAACTAAGGCACCCCACAGCTCTGAGG(+AAAAAA)GTACCTGGCAAGTGGCATAAGACCAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606180006_eb89 AGGCTTAGAACTTGAACTCGCCCTGACCTCTG(+AAAAAA)GATCCTGTTCTTCAAGGAAGCAAAACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606180004_pjxu TAAGGATTTGGTTTTGCTTCCTTGAAGAACAG(+AAAAAA)GATCCAGAGGTCAGGGCGAGTTCAAGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606180003_tryp GCCAGCCCAGAGCCGGGCCACTCCCCTTTCTG(+AAAAAA)GTACCAAGAAAAGCTTTCCTTGATGACCAGT NGG 10 8 17 8 24 0 100 5 10
    240606180002_qdlb AGGGCACTGGTCATCAAGGAAAGCTTTTCTTG(+AAAAAA)GTACCAGAAAGGGGAGTGGCCCGGCTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606180000_x0k4 TCAGTGCCGCTCAGCCTTGGCCCTGTGTGGCG(+AAAAAA)GATCCTGACCTAGAGAACAGGTGTGGGGACA NGG 10 8 17 8 24 0 100 5 10