Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181921_ljvu GCCAAGCTGGTCCTAGGAAGAACCCGAGAGAG(+AAAAAA)GATCCGGTGCTCCTTGTCCCATCAGATAGAT NGG 10 8 17 8 24 0 100 5 10
    240606181919_dmia AAAATAAATAATGCATTAATGCTTCTTCATTG(+AAAAAA)GTACCCCCCCCTTTTTTTTGAGACAGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606181917_quxd TAGTGAGACTCTGTCTCAAAAAAAAGGGGGGG(+AAAAAA)GTACCAATGAAGAAGCATTAATGCATTATTT NGG 10 8 17 8 24 0 100 5 10
    240606181915_zthq GGAATCTAGATTCCCCCAAGAGTTTAAGGAGG(+AAAAAA)GATCCAGAAATCCAAATTTTAAGAACTACAG NGG 10 8 17 8 24 0 100 5 10
    240606181914_n4jg CAATCCTGTAGTTCTTAAAATTTGGATTTCTG(+AAAAAA)GATCCCTCCTTAAACTCTTGGGGGAATCTAG NGG 10 8 17 8 24 0 100 5 10
    240606181912_6rcl AAGGCTTCCTTCCATTAATAAATATCATGGTG(+AAAAAA)GTACCCCCATGACTAAAAATTAGATTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606181909_we9o ATTCAGTGAAAATCTAATTTTTAGTCATGGGG(+AAAAAA)GTACCACCATGATATTTATTAATGGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606181907_4q8y GTCTTCTGGGAACAGCATTATACCATTGCAGG(+AAAAAA)GATCCTCTGCTGAAACTTTTTTAATACTATA NGG 10 8 17 8 24 0 100 5 10
    240606181906_fudo AAAAATATAGTATTAAAAAAGTTTCAGCAGAG(+AAAAAA)GATCCCTGCAATGGTATAATGCTGTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606181904_1ov7 ATGTACCCACATCCCCACAAGGGAACTTTAAG(+AAAAAA)GATCCACCCCTTAAGCCCCTCCCTCCTCTGT NGG 10 8 17 8 24 0 100 5 10