Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180132_75p4 ACCTTTATCCGACGAACACCAGCTTCCCCATG(+AAAAAA)GATCCCTATGGAGAGATGTGGTCATTACTGG NGG 10 8 17 8 24 0 100 5 10
    240606180131_263v AGGACTTTCAGATGGATTGATGAATCTTGCTG(+AAAAAA)GTACCAGTTTCACTACCAAAAGGTTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606180130_vyvf GCTCCTTTGACAACCTTTTGGTAGTGAAACTG(+AAAAAA)GTACCAGCAAGATTCATCAATCCATCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606180129_0s0c ACCCAGTCACTTTCCTACAATCCCACATAGAG(+AAAAAA)GATCCATTTTCTCTGGGAAGGAAGTTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606180127_ik5r ATACTGAGAAAACTTCCTTCCCAGAGAAAATG(+AAAAAA)GATCCTCTATGTGGGATTGTAGGAAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606180126_y28j GAGTATCTCTTTGAAAAGCCTCATTAAGCTGG(+AAAAAA)GTACCACTGCCTGTCATGGTGGTCAGTATAT NGG 10 8 17 8 24 0 100 5 10
    240606180125_ydts TATCCATATACTGACCACCATGACAGGCAGTG(+AAAAAA)GTACCCAGCTTAATGAGGCTTTTCAAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606180124_vngh GTAGGAGGCCACTAGCTACTTCCCAAAGTGAG(+AAAAAA)GATCCACCCAGAATCCCCCAGGCCCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240606180122_iegc GAGCTACAGTGGGGCCTGGGGGATTCTGGGTG(+AAAAAA)GATCCTCACTTTGGGAAGTAGCTAGTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606180121_ng8h ACTTCCTAGAAATGGGGCCCAGTAGAGTTCTG(+AAAAAA)GTACCCCTCCCTGGCTGAGCCCTCGGGGTCT NGG 10 8 17 8 24 0 100 5 10