Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180230_xtvc CGGTGACCCCAGGCTGGGGTTCCTGCTTGCTG(+AAAAAA)GATCCCTAGACTACTGCCTTAGCCGTGTGTT NGG 10 8 17 8 24 0 100 5 10
    240606180229_morx ACCCAAAAGATACGACTCTTGATCATCAATGG(+AAAAAA)GTACCCACTGTCCTAGTGCAAGTGAGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606180226_1r43 ATTGTCTTGTCTCACTTGCACTAGGACAGTGG(+AAAAAA)GTACCCATTGATGATCAAGAGTCGTATCTTT NGG 10 8 17 8 24 0 100 5 10
    240606180225_5ujs ATACATGGGGACCACCTCCCTCCCTTTCAATG(+AAAAAA)GATCCCAGCACCTCAGTTCCCCCCACTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606180224_dceh GACATCAATGTGACCTGACTCTAGGTCACTAG(+AAAAAA)GTACCTAAGAAAATGTGATAGGTATGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606180222_zccn AACGCAACTCCATACCTATCACATTTTCTTAG(+AAAAAA)GTACCTAGTGACCTAGAGTCAGGTCACATTG NGG 10 8 17 8 24 0 100 5 10
    240606180221_x4cr GTTTGTTTGTTTGAATAAACTAAAAATGCCTG(+AAAAAA)GTACCTAGGACGTAATCTAGAACTGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240606180219_hzcl CCCCAAAATGGCAGTTCTAGATTACGTCCTAG(+AAAAAA)GTACCAGGCATTTTTAGTTTATTCAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240606180217_egbg ATCTCAACAATGTGTGGGTCAAGCAGTAGCTG(+AAAAAA)GTACCTTACTCCTTCTCTTGTGCCAACCCCC NGG 10 8 17 8 24 0 100 5 10
    240606180216_8esk TATTTGGGGGTTGGCACAAGAGAAGGAGTAAG(+AAAAAA)GTACCAGCTACTGCTTGACCCACACATTGTT NGG 10 8 17 8 24 0 100 5 10