Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180329_8ztj GAAGGCACAGAGTTAATAGCACCAGGTCCTGG(+AAAAAA)GTACCGAGTGTAGGTGACAAAGCACGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606180327_xrga TCAGGGAAGTATGTGTCTCCCGGGGAGGGATG(+AAAAAA)GTACCTCCGTGGGCATCCCCCAAAGGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606180326_ix67 TGGTGGAGGGCCTTTGGGGGATGCCCACGGAG(+AAAAAA)GTACCATCCCTCCCCGGGAGACACATACTTC NGG 10 8 17 8 24 0 100 5 10
    240606180324_io52 CTCCACTTTATTTTTGAAATGCATACTTATTG(+AAAAAA)GTACCCTTTTGTTTCTTCCCTCACAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606180323_2vgd GGCAACATTTTTGTGAGGGAAGAAACAAAAGG(+AAAAAA)GTACCAATAAGTATGCATTTCAAAAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240606180321_sq33 AATAGTTTCTGTATTTTCTAATTATGAATAGG(+AAAAAA)GTACCCTTGATGTCTTCAAAGTTTTGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606180319_li49 TTAAGTGAGACAAAACTTTGAAGACATCAAGG(+AAAAAA)GTACCCTATTCATAATTAGAAAATACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606180317_cjrq TTCTATAAAACTGAGTTTGCTGAAACTTCCCG(+AAAAAA)GTACCCCACCATGCTTCTCTAATAAGCATGA NGG 10 8 17 8 24 0 100 5 10
    240606180315_ct4c TGAGCTCATGCTTATTAGAGAAGCATGGTGGG(+AAAAAA)GTACCGGGAAGTTTCAGCAAACTCAGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606180314_7tpu TCTCTGAGCAAAGAGGCTACAAGGGAGTTTTG(+AAAAAA)GATCCCGTGAGGCAGGCGCTACAGATGGAAC NGG 10 8 17 8 24 0 100 5 10