Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180259_12vw AACAGACTCACCCATTACCTCACATCCCTGTG(+AAAAAA)GATCCCAACACAGTAATGCTAAACATCAACT NGG 10 8 17 8 24 0 100 5 10
    240606180258_2lti CATTTTGGGAGCGTTTTTGACTAGGCAACTTG(+AAAAAA)GATCCATTCCTTTCCAGATGTTGAAGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606180257_dywm GTTATAAGCCCTTCAACATCTGGAAAGGAATG(+AAAAAA)GATCCAAGTTGCCTAGTCAAAAACGCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240606180255_hf32 TTAGAAGACCAATTTAACCAGGCTCACACATG(+AAAAAA)GTACCAAATACCAGTTTTGTAGCTTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606180254_rpwk AAGCAGGAAGAAAGCTACAAAACTGGTATTTG(+AAAAAA)GTACCATGTGTGAGCCTGGTTAAATTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606180252_cg2h GGTGTTATCTGAAGCAGGGCAGTGTGGTTTAG(+AAAAAA)GATCCTTAGCACCACAAAAAACAAAGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606180251_xgp7 ACTAGGCTGGCTTTGTTTTTTGTGGTGCTAAG(+AAAAAA)GATCCTAAACCACACTGCCCTGCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240606180250_dsuz AAATTATAAAGCCCCATAAGCAAGTCACTTTG(+AAAAAA)GTACCATTCCTGAGAAAGAAGTTTACATATA NGG 10 8 17 8 24 0 100 5 10
    240606180248_bns1 TTTATTATATGTAAACTTCTTTCTCAGGAATG(+AAAAAA)GTACCAAAGTGACTTGCTTATGGGGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606180246_lfz8 ATACTCAAGTCTCCTATTTGTTCAACCAAGGG(+AAAAAA)GATCCAATAAGATGTACTAGCGAGTATTTAT NGG 10 8 17 8 24 0 100 5 10