Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180245_cava TGCTCATAAATACTCGCTAGTACATCTTATTG(+AAAAAA)GATCCCCTTGGTTGAACAAATAGGAGACTTG NGG 10 8 17 8 24 0 100 5 10
    240606180242_2v6g CCAAATATTTATATTTACATACATTTCAAAGG(+AAAAAA)GATCCAGTGATTGATATAAGATTCAGTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606180241_581h CCATGTTAAACTGAATCTTATATCAATCACTG(+AAAAAA)GATCCCTTTGAAATGTATGTAAATATAAATA NGG 10 8 17 8 24 0 100 5 10
    240606180240_gxjm AATGCCTGCAAACATTCAGACTAATCAACGTG(+AAAAAA)GTACCGTAATTTCTTTTAATACATCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240606180239_e596 AAACTATTATTGATGTATTAAAAGAAATTACG(+AAAAAA)GTACCACGTTGATTAGTCTGAATGTTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606180237_3im6 CACACTTGCCTGGCGTGTAAGGCACTGGGTTG(+AAAAAA)GATCCTCAGTACTTTGAAGAAAAGCAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606180236_byop TCAGGGCTTTTGCTTTTCTTCAAAGTACTGAG(+AAAAAA)GATCCAACCCAGTGCCTTACACGCCAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240606180235_31wx ACTGTCACGCTGCACACGTTTTACTGCTTGAG(+AAAAAA)GATCCTACATCTGTGTTTTAGGTCTTTGATG NGG 10 8 17 8 24 0 100 5 10
    240606180233_3l8v GGTCACATCAAAGACCTAAAACACAGATGTAG(+AAAAAA)GATCCTCAAGCAGTAAAACGTGTGCAGCGTG NGG 10 8 17 8 24 0 100 5 10
    240606180232_w97w TGCAAAACACACGGCTAAGGCAGTAGTCTAGG(+AAAAAA)GATCCAGCAAGCAGGAACCCCAGCCTGGGGT NGG 10 8 17 8 24 0 100 5 10