Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180345_7ygx TGGCTCATCCTCTACATCAGCCATAGCTCGTG(+AAAAAA)GATCCGACCACTAACAAATCTCAAGCCTGAT NGG 10 8 17 8 24 0 100 5 10
    240606180343_r9z7 ACCCCATGCTGCTCTCAAAAGGCTAAACCCTG(+AAAAAA)GTACCCGTGGTGGCCCTTGCTCCAGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606180340_n30f GCCTCCCTGCACTGGAGCAAGGGCCACCACGG(+AAAAAA)GTACCAGGGTTTAGCCTTTTGAGAGCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240606180339_sm79 TTCCCACCAAGCTAGGGCGTGGCTAGATAAAG(+AAAAAA)GTACCTCTGACTTGGGTTTCTAACAGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606180338_d7ap ATGTATCACGCTGTTAGAAACCCAAGTCAGAG(+AAAAAA)GTACCTTTATCTAGCCACGCCCTAGCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606180336_cq01 AGTTTAAACCTACATACCCAGTTTCCTTTCAG(+AAAAAA)GTACCTTGACCTGCTTCCAGGGGAACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606180335_zhc9 GCTATTAAAAGTTCCCCTGGAAGCAGGTCAAG(+AAAAAA)GTACCTGAAAGGAAACTGGGTATGTAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606180334_qfyo TTCCAAGGATGGAACTCAGGTGGTTAGTATTG(+AAAAAA)GTACCAGGAACCTAAACTTACCCTTTGAACC NGG 10 8 17 8 24 0 100 5 10
    240606180332_xfmv GGGTTGGTTCAAAGGGTAAGTTTAGGTTCCTG(+AAAAAA)GTACCAATACTAACCACCTGAGTTCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606180331_o7q3 AATGCCCTTTCGTGCTTTGTCACCTACACTCG(+AAAAAA)GTACCCAGGACCTGGTGCTATTAACTCTGTG NGG 10 8 17 8 24 0 100 5 10