Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606180119_cz6c AGGGGAGACCCCGAGGGCTCAGCCAGGGAGGG(+AAAAAA)GTACCAGAACTCTACTGGGCCCCATTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240606180117_izlu AACGATATACTTACTAAATGCACTTAAACCAG(+AAAAAA)GTACCAAGGAGTAGGACAGAACTGGAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606180115_6a5j CACTCAGCTTTCCAGTTCTGTCCTACTCCTTG(+AAAAAA)GTACCTGGTTTAAGTGCATTTAGTAAGTATA NGG 10 8 17 8 24 0 100 5 10
    240606180114_yc86 CAAAAGCAGAGACTTGGTGAGCTGCCTGGTGG(+AAAAAA)GTACCTGGAAGAGGAGAGCAGAATTAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606180112_u2vd GACTCATTCTTAATTCTGCTCTCCTCTTCCAG(+AAAAAA)GTACCCACCAGGCAGCTCACCAAGTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606180110_5bgb TAGGGTGTGAGGTCTCAGGAGGGCTGTTGGGG(+AAAAAA)GTACCCAGTGAGGCTCAAGCAGGAGTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606180108_p8y8 TTCCCTCTACACTCCTGCTTGAGCCTCACTGG(+AAAAAA)GTACCCCCAACAGCCCTCCTGAGACCTCACA NGG 10 8 17 8 24 0 100 5 10
    240606180106_f7zl AAGGGAGAGCTGAATGGAACAGAGCAAGGGAG(+AAAAAA)GTACCCAGACGCGATGGGACCTCTCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606180105_7667 AGCGAGGGAAGGAGAGGTCCCATCGCGTCTGG(+AAAAAA)GTACCTCCCTTGCTCTGTTCCATTCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606180104_t1rj TCTCCAAGAGATATTTGCATATACATTAACAG(+AAAAAA)GTACCAGTAACCCGTGTGTGTCTCCAATTAA NGG 10 8 17 8 24 0 100 5 10