Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175658_p85i TCATTTTTTTTTTTTTTTAATTTATTCTCTGG(+AAAAAA)GATCCCTTAGTAACCTGGCATTGCTCATTGC NGG 10 8 17 8 24 0 100 5 10
    240606175656_17u5 CCTTAGGAAAACAACAGAAAGCACACAGTTTG(+AAAAAA)GTACCCGGGGAAAGTCCTAAATTGTGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606175653_qk75 ATGTCAGGAACACAATTTAGGACTTTCCCCGG(+AAAAAA)GTACCAAACTGTGTGCTTTCTGTTGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606175651_lh1w TATAGAAAATATCAGCAGAGCTTGTCTTCCTG(+AAAAAA)GTACCACATTTTAAGTGACAAGGTTTGTATA NGG 10 8 17 8 24 0 100 5 10
    240606175650_u49i AAACATATACAAACCTTGTCACTTAAAATGTG(+AAAAAA)GTACCAGGAAGACAAGCTCTGCTGATATTTT NGG 10 8 17 8 24 0 100 5 10
    240606175649_acc3 GATGAGAAGGGTAAGTCTTCCCAGAGGAAGTG(+AAAAAA)GTACCAATACCTGTATGCTGGGCATCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606175648_63v2 TCTTCCTCAGGATGCCCAGCATACAGGTATTG(+AAAAAA)GTACCACTTCCTCTGGGAAGACTTACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606175646_lqep TGTGTACTATGGAGATGCATATTCTTTCCAAG(+AAAAAA)GTACCTTTCTCACACGAATTTCACAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240606175645_7ge6 GCTCTGAGATCTGTGAAATTCGTGTGAGAAAG(+AAAAAA)GTACCTTGGAAAGAATATGCATCTCCATAGT NGG 10 8 17 8 24 0 100 5 10
    240606175644_43j6 CACCTGCAGTAATGTCCTAACTGTGCATTCTG(+AAAAAA)GATCCTCCAAGAGGACGAGCAAGGGAGTGAG NGG 10 8 17 8 24 0 100 5 10