Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175712_146m TAAGGCAGACTTAATTTGTCAGATCGTAACAG(+AAAAAA)GATCCCAAAAGACAGAGCAGCTGCTCACAAC NGG 10 8 17 8 24 0 100 5 10
    240606175711_12pd GTTGACCTGGTGATATTATCCTGGAGTCTCTG(+AAAAAA)GATCCAGCCACGCTTCATCTCCAACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240606175709_w392 GGAAAGGATGAGTTGGAGATGAAGCGTGGCTG(+AAAAAA)GATCCAGAGACTCCAGGATAATATCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240606175708_18vk GAGCTACAAACCATGTTTGTAAATGCTCAGCG(+AAAAAA)GATCCTTTCAACACACTCTAAGATACTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606175707_06vd TGTTCTTTGAGTATCTTAGAGTGTGTTGAAAG(+AAAAAA)GATCCGCTGAGCATTTACAAACATGGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606175704_kh6s AATAGCTTATGGCTCAGAATATCTAAGCCCTG(+AAAAAA)GTACCTTGAAATGCTCTCCTTTCATTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240606175703_82kz AAAAAGTAAGAATGAAAGGAGAGCATTTCAAG(+AAAAAA)GTACCAGGGCTTAGATATTCTGAGCCATAAG NGG 10 8 17 8 24 0 100 5 10
    240606175702_3x3x CTACTAGCAACAGTATTTCTGTAGTCCGCTCG(+AAAAAA)GATCCTATAAGGAGTATGTGTGGAAACTCAA NGG 10 8 17 8 24 0 100 5 10
    240606175701_uubt TCCTCTTGAGTTTCCACACATACTCCTTATAG(+AAAAAA)GATCCGAGCGGACTACAGAAATACTGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606175659_jfry CATGAGCAATGAGCAATGCCAGGTTACTAAGG(+AAAAAA)GATCCCAGAGAATAAATTAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10