Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175557_b1xv ACGGCCCACCAGACCCAGATTTTTTTCTCTGG(+AAAAAA)GTACCAAGGATCCCAGCTCAGGTCCTTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606175556_8s7b GCTCGCTGTGAAAACACAAGGACCTGAGCTGG(+AAAAAA)GATCCTTGGTACCCAGAGAAAAAAATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606175554_u8ex CCAGACCCAGATTTTTTTCTCTGGGTACCAAG(+AAAAAA)GATCCCAGCTCAGGTCCTTGTGTTTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606175551_r1nb TAGCAAACCAAAAAACACCCCTTTTCCCAAGG(+AAAAAA)GATCCTACATCTCTAGCATTTGTGAAACCTG NGG 10 8 17 8 24 0 100 5 10
    240606175550_oajc GACCCCAGGTTTCACAAATGCTAGAGATGTAG(+AAAAAA)GATCCCTTGGGAAAAGGGGTGTTTTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606175548_gqxi ACTCCCCCATAGGGTCAAACGGCTCAACCAAG(+AAAAAA)GTACCTGGCCAGGACACAGTCCTCATGCTAG NGG 10 8 17 8 24 0 100 5 10
    240606175546_v72k AGGAGCTAGCATGAGGACTGTGTCCTGGCCAG(+AAAAAA)GTACCTTGGTTGAGCCGTTTGACCCTATGGG NGG 10 8 17 8 24 0 100 5 10
    240606175545_h79y CGGTCTGGAGCCAGCGGAGAAGGAGGAGATTG(+AAAAAA)GATCCCTCAGAAGGGCTCCCCGTGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606175544_fwx7 GGGGTGGGGCTGCACGGGGAGCCCTTCTGAGG(+AAAAAA)GATCCAATCTCCTCCTTCTCCGCTGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606175542_9f0r AGATTTTGAGATCACATCGTTCTCCCCTCAAG(+AAAAAA)GATCCCCTGATTAGTGGGAGACTAAGACCTG NGG 10 8 17 8 24 0 100 5 10