Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175540_3mxm GGGGGCAGGTCTTAGTCTCCCACTAATCAGGG(+AAAAAA)GATCCTTGAGGGGAGAACGATGTGATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240606175539_xp7f TGAGAGAAGGGCTGGGAGCCAGCCAAGGGATG(+AAAAAA)GTACCTTTGTTGTGACGGGGCTACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606175538_op30 GCTGAGAAGGGTGTAGCCCCGTCACAACAAAG(+AAAAAA)GTACCATCCCTTGGCTGGCTCCCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606175536_z73r AGAAGTCCAGATACTCCATTCTTGAGTGTCAG(+AAAAAA)GATCCCAGGCAAAACTGGCAATACAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240606175534_2fua GTTTCCTGGATTGTATTGCCAGTTTTGCCTGG(+AAAAAA)GATCCTGACACTCAAGAATGGAGTATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606175533_0orv GTGCTTGCTGCCAGGTTTGACCACGTGAGTTG(+AAAAAA)GATCCCTGGAACCCACATGGTGGAAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606175530_efdi CAATTCTCTCTTTCCACCATGTGGGTTCCAGG(+AAAAAA)GATCCAACTCACGTGGTCAAACCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606175529_i69u GTGGTCATTGAGTGCCACCAGTACATCTGATG(+AAAAAA)GATCCAGTTGGCACGTCATGACAGATTGACT NGG 10 8 17 8 24 0 100 5 10
    240606175528_27ps ACACAAGTCAATCTGTCATGACGTGCCAACTG(+AAAAAA)GATCCATCAGATGTACTGGTGGCACTCAATG NGG 10 8 17 8 24 0 100 5 10
    240606175526_v8r7 AGGACGGAGCCTAGGACTCTGTACCTGCCAAG(+AAAAAA)GATCCCAGAGCCCAAGTATGTGGGGCTTTGG NGG 10 8 17 8 24 0 100 5 10