Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606175223_bpmf GTCTATCTTTTAAAAGATGTGGCTACCTGTGG(+AAAAAA)GATCCTTTGCAGAGTCCAGGCTTATGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606175222_a83r AGTGGTGAAGTGTTCTTACCACCCCAGCTCAG(+AAAAAA)GATCCTTGAAGCCTGGGGTTGTGAGAAAACT NGG 10 8 17 8 24 0 100 5 10
    240606175220_swmm AATTGAGTTTTCTCACAACCCCAGGCTTCAAG(+AAAAAA)GATCCTGAGCTGGGGTGGTAAGAACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240606175218_lvd8 TGGCTCATCTCCTTTTGTCTGGACATCCTGGG(+AAAAAA)GTACCAAGAAATACACCTGAGAAGAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240606175217_kz2m TGTGTGTTTGTTCTTCTCAGGTGTATTTCTTG(+AAAAAA)GTACCCCAGGATGTCCAGACAAAAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606175215_v3z8 GTCCTTGTAATGAGAATTCCCTCTCTTTGAAG(+AAAAAA)GTACCCAGCCTAGCCTCCAGGCCTCAACATT NGG 10 8 17 8 24 0 100 5 10
    240606175214_jx6c CGCCAAATGTTGAGGCCTGGAGGCTAGGCTGG(+AAAAAA)GTACCTTCAAAGAGAGGGAATTCTCATTACA NGG 10 8 17 8 24 0 100 5 10
    240606175213_wpgi TCTGATTCAAGAAATAGCCTGGGTCAGAAGAG(+AAAAAA)GTACCCCAGGTAAAATTGAGAGAGCATTAGG NGG 10 8 17 8 24 0 100 5 10
    240606175210_meag TTTTGCCTAATGCTCTCTCAATTTTACCTGGG(+AAAAAA)GTACCTCTTCTGACCCAGGCTATTTCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606175209_pswj TTTTGCCAATGAGTCTGAGGCTGTGGTCTCTG(+AAAAAA)GATCCAGCCATGATGTTTTTATATGTTTAAT NGG 10 8 17 8 24 0 100 5 10