Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173301_2w2m CACACACACACAGAGTCCTCGATTGAGGGGCG(+AAAAAA)GATCCTCTATGTGGGCGGTTCCAGAACTTCC NGG 10 8 17 8 24 0 100 5 10
    240606173300_x59e AAGAAGAAGAAGAAAAAAAGAATAAGACACTG(+AAAAAA)GATCCTAAAGAAAACCGTGCCCAAGCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606173259_79w7 CGGGGTCTAGGCTTGGGCACGGTTTTCTTTAG(+AAAAAA)GATCCAGTGTCTTATTCTTTTTTTCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606173257_z7jq GGTTGCAACCTTGAGAAAGCTCCAGTTACTGG(+AAAAAA)GTACCCTGCTTATCGCTGCCTCCCAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240606173256_eeox CAGCAAAATCCTGGGAGGCAGCGATAAGCAGG(+AAAAAA)GTACCCAGTAACTGGAGCTTTCTCAAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606173254_8l50 ACCACTTGGTGGCTCACAACCATCTGTAATGG(+AAAAAA)GATCCAATGCCCTCTTTAATGTGTCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606173253_up7t CTCTGTCTTCAGACACATTAAAGAGGGCATTG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAA NGG 10 8 17 8 24 0 100 5 10
    240606173251_ukwc GTTCATTCTGTCAGGTTCTTTCATACACAAGG(+AAAAAA)GTACCTTCAGAAGAAACGGGCACCTATAACA NGG 10 8 17 8 24 0 100 5 10
    240606173250_ep2p TTCTTTGTTATAGGTGCCCGTTTCTTCTGAAG(+AAAAAA)GTACCCTTGTGTATGAAAGAACCTGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240606173248_7cny GCCTTAGGAGTGAAGACAGCCAGGAGAGGCTG(+AAAAAA)GTACCGGCCCGCCTCCCTCACGGAGTCTCCT NGG 10 8 17 8 24 0 100 5 10