Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173214_dkzj ACTGAGTGTCCTCACCAACTGTACCATCAGTG(+AAAAAA)GTACCAAAACTGACTGGTTTTAAAAAACACG NGG 10 8 17 8 24 0 100 5 10
    240606173213_08ug ATCCACGTGTTTTTTAAAACCAGTCAGTTTTG(+AAAAAA)GTACCACTGATGGTACAGTTGGTGAGGACAC NGG 10 8 17 8 24 0 100 5 10
    240606173212_b380 ATAAGGGCCCTGGACAGGCACAGATACTCTTG(+AAAAAA)GATCCCCAGTGGAGATGGATAAGAACCTCAA NGG 10 8 17 8 24 0 100 5 10
    240606173210_xs0t CCTCTTTGAGGTTCTTATCCATCTCCACTGGG(+AAAAAA)GATCCAAGAGTATCTGTGCCTGTCCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606173207_i7ah GGATGGACCATCCAGAGACTACCCCACCCGGG(+AAAAAA)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606173205_dnr3 AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+AAAAAA)GATCCCCGGGTGGGGTAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606173204_t2ry CTTGCTGCCAGGAGAGCTTGCTTGGGTATCAG(+AAAAAA)GTACCATACCCCTACTGTGCAGAGGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606173203_or4k TCTTCCCAGAGCCTCTGCACAGTAGGGGTATG(+AAAAAA)GTACCTGATACCCAAGCAAGCTCTCCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240606173201_m2u0 ATGACATGATCACCGCCTCACAGTCAGTGATG(+AAAAAA)GTACCTTTGTTTTAGTTATGACCAGATAGCT NGG 10 8 17 8 24 0 100 5 10
    240606173200_0w4x ACCCCAGCTATCTGGTCATAACTAAAACAAAG(+AAAAAA)GTACCATCACTGACTGTGAGGCGGTGATCAT NGG 10 8 17 8 24 0 100 5 10