Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173123_n7e4 ACAGTCTGGGTCTTTATTGATTGACAGTCTGG(+AAAAAA)GTACCACCACACACACAGACTTCAGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606173121_ij7f TTAAGAAATACCTGAAGTCTGTGTGTGTGGTG(+AAAAAA)GTACCCAGACTGTCAATCAATAAAGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606173120_ugzn GGGAGTCGTCTGCTTCACATGGATATGCGACG(+AAAAAA)GTACCTTCTGACAGGGGGTTTTCACCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606173119_rp2z GTTTTCAAGGGGTGAAAACCCCCTGTCAGAAG(+AAAAAA)GTACCGTCGCATATCCATGTGAAGCAGACGA NGG 10 8 17 8 24 0 100 5 10
    240606173117_rc4o GAAGATCTCCCTAGAGGCCACTCCTTTAGGAG(+AAAAAA)GTACCAGGAACGCTTTCTGGTGTTGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606173115_w2j2 AGCAGCCCTAACAACACCAGAAAGCGTTCCTG(+AAAAAA)GTACCTCCTAAAGGAGTGGCCTCTAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606173114_lllx ATGCCAAGCTTAACATACGCGGACCCTCACAG(+AAAAAA)GATCCCTAGATCAACCACCCTGAACTGAACT NGG 10 8 17 8 24 0 100 5 10
    240606173113_1eay GTTCCAGTTCAGTTCAGGGTGGTTGATCTAGG(+AAAAAA)GATCCTGTGAGGGTCCGCGTATGTTAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606173111_dl4w GAAGATCTCCCTAGAGGCCACTCCTTTAGGAG(+AAAAAA)GTACCAGGAACGCTTTCTGGTGTTGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606173109_34q3 AGCAGCCCTAACAACACCAGAAAGCGTTCCTG(+AAAAAA)GTACCTCCTAAAGGAGTGGCCTCTAGGGAGA NGG 10 8 17 8 24 0 100 5 10