Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173035_kcv0 GAGGCCAAAATTAGTTTTTGCTACTTGATTTG(+AAAAAA)GTACCTTAGGGTTTGGGCTCCTCTGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606173034_8ft4 CAAGAAAAAACCAGAGGAGCCCAAACCCTAAG(+AAAAAA)GTACCAAATCAAGTAGCAAAAACTAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240606173032_2zu3 GATAGCAAAACAAAGGCACCTCTACACTCCAG(+AAAAAA)GTACCCCACATACATAAACCATTGTTGATCC NGG 10 8 17 8 24 0 100 5 10
    240606173030_n42n CCTACGGATCAACAATGGTTTATGTATGTGGG(+AAAAAA)GTACCTGGAGTGTAGAGGTGCCTTTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606173028_86h5 ATTGTCATGGGGCTCAGGGATACAGATAACAG(+AAAAAA)GATCCTAGAAGCAGGCGTGATATTCCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606173027_uz2h CCTGGTCCTTGGAATATCACGCCTGCTTCTAG(+AAAAAA)GATCCTGTTATCTGTATCCCTGAGCCCCATG NGG 10 8 17 8 24 0 100 5 10
    240606173026_v0ji GAGGCCAGGCAGGGCCTTGGGCCCAGGAACAG(+AAAAAA)GTACCTTGAGAGTGAGAGCTACCCATTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606173025_36s6 GCCACAGGCAATGGGTAGCTCTCACTCTCAAG(+AAAAAA)GTACCTGTTCCTGGGCCCAAGGCCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606173024_t8uc GCGTCCTCTGGGACCTTCTTAGATGCTGTAAG(+AAAAAA)GTACCTACATACAGACTAAATGTGCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606173023_fbqu TCAAGGTGCTTGCACATTTAGTCTGTATGTAG(+AAAAAA)GTACCTTACAGCATCTAAGAAGGTCCCAGAG NGG 10 8 17 8 24 0 100 5 10