Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172935_r5g1 TACAAGATCAACTGCTGGCTCCATAGCTTCTG(+AAAAAA)GATCCAGTGTCCCTTTCCCTTCAGAAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606172934_umjw GTGTTCAGGCTTCTGAAGGGAAAGGGACACTG(+AAAAAA)GATCCAGAAGCTATGGAGCCAGCAGTTGATC NGG 10 8 17 8 24 0 100 5 10
    240606172931_ve86 CTCCTGTGGCCTCTCCAGCAGGAGACCCACAG(+AAAAAA)GATCCCATGCAATGGCTGCTTTGCTCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606172930_5mv3 GCCTACTGAGGAGCAAAGCAGCCATTGCATGG(+AAAAAA)GATCCTGTGGGTCTCCTGCTGGAGAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606172929_r02p GCTAAATGGATGCTTCAGTCCATGGCTGAGAG(+AAAAAA)GTACCGCCTGCACCAGCAAAGTGGAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606172927_glmj AGCTTCAGCACTCCACTTTGCTGGTGCAGGCG(+AAAAAA)GTACCTCTCAGCCATGGACTGAAGCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240606172926_04u2 TAGCTTTGTTACATCCTAGCAAGATATTCCTG(+AAAAAA)GATCCCTGCTGCGCATTCTGATGTGAATACC NGG 10 8 17 8 24 0 100 5 10
    240606172924_y553 ACCTTGGTATTCACATCAGAATGCGCAGCAGG(+AAAAAA)GATCCAGGAATATCTTGCTAGGATGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240606172923_ysll ATATTTTTTCACCTAATCTCGGGGTCTTACTG(+AAAAAA)GTACCCTCACCCAAGTGCCTATGAAGCACGA NGG 10 8 17 8 24 0 100 5 10
    240606172921_ahjt GAACCTCGTGCTTCATAGGCACTTGGGTGAGG(+AAAAAA)GTACCAGTAAGACCCCGAGATTAGGTGAAAA NGG 10 8 17 8 24 0 100 5 10