Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173022_nof2 TTGTAAACCACTAAGACACTGTTAATCACACG(+AAAAAA)GATCCTAAGCAGCTGATGGTATCGCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606173021_zno1 ACAGCGGCAGTGCGATACCATCAGCTGCTTAG(+AAAAAA)GATCCGTGTGATTAACAGTGTCTTAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606173018_6nwq CTCGAAGAGTTTAGCATTTATTAACTCCCAGG(+AAAAAA)GATCCTAAACTCTTTGCTGATGGAATTGCCC NGG 10 8 17 8 24 0 100 5 10
    240606173017_02am AGATTGGGCAATTCCATCAGCAAAGAGTTTAG(+AAAAAA)GATCCCTGGGAGTTAATAAATGCTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240606173015_g2o9 CTCGCTCAAACAGTCCTACTCACAAACAGGAG(+AAAAAA)GTACCATATCCTACATCCTTTGGCTTTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606173014_1wyd CACTGTGGAAAAGCCAAAGGATGTAGGATATG(+AAAAAA)GTACCTCCTGTTTGTGAGTAGGACTGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606173012_wp1v TGACTGAAGACAAGGTGGATCTTAGCTAATAG(+AAAAAA)GATCCTCCCAGGGTTGTCAAGCGGGATCTGG NGG 10 8 17 8 24 0 100 5 10
    240606173011_s8s5 ACTCTCCAGATCCCGCTTGACAACCCTGGGAG(+AAAAAA)GATCCTATTAGCTAAGATCCACCTTGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606173009_tl60 AAGTCTGCGCACACACCTTCTCTGGTCTCTGG(+AAAAAA)GATCCAGGAGGTCAGCGAGCATCCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606173007_llsn TAGGAAGGGAGGGGATGCTCGCTGACCTCCTG(+AAAAAA)GATCCCAGAGACCAGAGAAGGTGTGTGCGCA NGG 10 8 17 8 24 0 100 5 10