Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606173052_ipnh AGATTAAAACCCTCTGCAAGCGCACAGCCTTG(+AAAAAA)GTACCGCCTCTCATCTCAGTTAACAGTTAGA NGG 10 8 17 8 24 0 100 5 10
    240606173051_alx1 TGTCATCTAACTGTTAACTGAGATGAGAGGCG(+AAAAAA)GTACCAAGGCTGTGCGCTTGCAGAGGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606173048_e2u7 ACAGTGTGCTCCTGTCTCTGTCACAGTTCCCG(+AAAAAA)GATCCCTCAGCAGCCCTGTGTGTTGATAAAG NGG 10 8 17 8 24 0 100 5 10
    240606173046_j7bw GCAAACTTTATCAACACACAGGGCTGCTGAGG(+AAAAAA)GATCCGGGAACTGTGACAGAGACAGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606173045_06hm AGTACAAGGGCCCTCAGAAGGTGGAGGCATTG(+AAAAAA)GATCCCCTGGAGCTGGAGTTATGGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240606173043_ilv0 CGGCAGCCATATCCATAACTCCAGCTCCAGGG(+AAAAAA)GATCCAATGCCTCCACCTTCTGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606173041_j6j2 AGCCACAGCTGGCTTTCCACACAGGTTCTAGG(+AAAAAA)GATCCAAACAGGGCCTCAGGTGTATTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606173040_n7dp AACCCTGACTAATACACCTGAGGCCCTGTTTG(+AAAAAA)GATCCCTAGAACCTGTGTGGAAAGCCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606173038_kgre CATGTTTATAAAATAGCACGTACTGAAAACTG(+AAAAAA)GTACCACCCGTGACTGTATAAAGAAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606173037_u5ru AGTAATCTACCTTCTTTATACAGTCACGGGTG(+AAAAAA)GTACCAGTTTTCAGTACGTGCTATTTTATAA NGG 10 8 17 8 24 0 100 5 10