Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172834_x8w3 TTTTCCCTGAGTGCCAACCTTAAAGATGGGTG(+AAAAAA)GTACCAACTTCACTTGGCTAGTAGAGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240606172833_u6iw CCTCAGCTAGCTCTACTAGCCAAGTGAAGTTG(+AAAAAA)GTACCACCCATCTTTAAGGTTGGCACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606172831_a2gj TTTTTTGTCAATCATATGATTTTCTGAAGAAG(+AAAAAA)GTACCACCATGCTATGGTGCTGTTTGAATCC NGG 10 8 17 8 24 0 100 5 10
    240606172830_8spx CCCATGGATTCAAACAGCACCATAGCATGGTG(+AAAAAA)GTACCTTCTTCAGAAAATCATATGATTGACA NGG 10 8 17 8 24 0 100 5 10
    240606172829_jxuu AAGAAGAAAGCAAGACACAGGGCTGCACTTTG(+AAAAAA)GATCCACGTTTAATGAAAAAGAAGTCACGTG NGG 10 8 17 8 24 0 100 5 10
    240606172827_ocpe CCAGTCACGTGACTTCTTTTTCATTAAACGTG(+AAAAAA)GATCCAAAGTGCAGCCCTGTGTCTTGCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606172826_1dse CCCCTGGAGCTGGAGTTACAGGAGGTTGTGAG(+AAAAAA)GTACCCGGTGTGGGTGCTGGGAGCCAAACTT NGG 10 8 17 8 24 0 100 5 10
    240606172824_xcgm GACCCAAGTTTGGCTCCCAGCACCCACACCGG(+AAAAAA)GTACCTCACAACCTCCTGTAACTCCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606172822_wohd TTATAACCTTCCAGCATTGGTTCTTGACTTTG(+AAAAAA)GATCCAAAGCCCCAGTAGAAGAGAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240606172821_q24g TCTGCTCCATCTTCTCTTCTACTGGGGCTTTG(+AAAAAA)GATCCAAAGTCAAGAACCAATGCTGGAAGGT NGG 10 8 17 8 24 0 100 5 10