Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172820_cd4u GACCTCCAAAGGTACAGGTCAGAATAGGGCAG(+AAAAAA)GATCCCTTGTGAACTACCCTACCATGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606172818_3ku9 TCAGCACCCACATGGTAGGGTAGTTCACAAGG(+AAAAAA)GATCCTGCCCTATTCTGACCTGTACCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606172816_i4rf GGCTGATACACTGAGTGCAAGAGTACTGGAGG(+AAAAAA)GATCCAGTGAGTCAAACCAGTAAGCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606172815_5npu CACACCTCACTGCTTACTGGTTTGACTCACTG(+AAAAAA)GATCCCTCCAGTACTCTTGCACTCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606172814_pe61 CATTATCTTTGACAATTTAAACAAGATCAAAG(+AAAAAA)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240606172812_ce5h CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+AAAAAA)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606172811_pbea CATTATCTTTGACAATTTAAACAAGATCAAAG(+AAAAAA)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240606172809_eelr CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+AAAAAA)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606172808_y8gw GTTTTCTGAAGTCATGGATTAAGTGATCTGCG(+AAAAAA)GATCCACCCAGGCACAGTCAGATCAAAGCGT NGG 10 8 17 8 24 0 100 5 10
    240606172806_lg80 TTGCAACGCTTTGATCTGACTGTGCCTGGGTG(+AAAAAA)GATCCGCAGATCACTTAATCCATGACTTCAG NGG 10 8 17 8 24 0 100 5 10