Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172714_j3a4 AAAAATCACCTGTGGAAGCACAAAATTCCATG(+AAAAAA)GATCCAGAGTGGAGAGTGTTCTGTTCTATGC NGG 10 8 17 8 24 0 100 5 10
    240606172712_ow15 TTAATGCATAGAACAGAACACTCTCCACTCTG(+AAAAAA)GATCCATGGAATTTTGTGCTTCCACAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606172711_8m6v TGTTAGCGAAACACCTTTAAAGATTTATAAGG(+AAAAAA)GATCCCAGGTAATTAGTATATATGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240606172708_kgrv CAATAAAAATTTCATATATACTAATTACCTGG(+AAAAAA)GATCCCTTATAAATCTTTAAAGGTGTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240606172707_moli GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+AAAAAA)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240606172705_2ii9 TGTTTCGTGTAATACTGCCACACCCATAAGAG(+AAAAAA)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606172704_fqhl GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+AAAAAA)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240606172702_i2jp TGTTTCGTGTAATACTGCCACACCCATAAGAG(+AAAAAA)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606172700_e7s4 TTCAAGCAACATATAAAATGAAGAGTTTCCTG(+AAAAAA)GTACCTATCATAGTATTTACATTCCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240606172659_5p1w TTTGAGATAAGGGAATGTAAATACTATGATAG(+AAAAAA)GTACCAGGAAACTCTTCATTTTATATGTTGC NGG 10 8 17 8 24 0 100 5 10