Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172626_ouvw CATAAATCTGGGAAGAAATGGCGGTGCAGTGG(+AAAAAA)GATCCCAGAAACGAAGCCTCCACCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606172625_58pi GAAAAACAATGACTGGAATATCATTGGAGATG(+AAAAAA)GATCCCAGGTAGGGGTTACAATTAGCCATGG NGG 10 8 17 8 24 0 100 5 10
    240606172623_faer ACAAACCATGGCTAATTGTAACCCCTACCTGG(+AAAAAA)GATCCATCTCCAATGATATTCCAGTCATTGT NGG 10 8 17 8 24 0 100 5 10
    240606172621_l9xr CACAGCACAAACTACCAAATTTTGATTTTTTG(+AAAAAA)GATCCCAGCAATAATTTTTAATAGTTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606172620_61qp CCAGTTTGAAAACTATTAAAAATTATTGCTGG(+AAAAAA)GATCCAAAAAATCAAAATTTGGTAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606172619_52e0 ACCCATCTCAGGTTATAGTCCACATAAGCGTG(+AAAAAA)GATCCTACATGTTTCTTGATTAGGATAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606172617_7n1w TAGGTAGACTATCCTAATCAAGAAACATGTAG(+AAAAAA)GATCCACGCTTATGTGGACTATAACCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606172615_9m6h TGCCTGTACCTTCAAATGTAAAGTAAGCCACG(+AAAAAA)GTACCTTGTGAGACATGTGTTCCTTGTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606172614_nkb1 CCAAGGAACACAAGGAACACATGTCTCACAAG(+AAAAAA)GTACCGTGGCTTACTTTACATTTGAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240606172612_biy8 AGGTGACGGTCTGCTCATTTGTCAATGGGATG(+AAAAAA)GTACCTTCCTTTCTCTTCATGGATTGCATCC NGG 10 8 17 8 24 0 100 5 10