Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172536_0x4y GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606172535_kptf AGTGATGACTCAGATGACGACACTTTATAATG(+AAAAAA)GTACCTGGTTGGTGGACAACTCACACAAACT NGG 10 8 17 8 24 0 100 5 10
    240606172533_737c AACACAGTTTGTGTGAGTTGTCCACCAACCAG(+AAAAAA)GTACCATTATAAAGTGTCGTCATCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606172531_jc7r CCAGCTGGCTGGATGTGCCCAGATCCTCCTTG(+AAAAAA)GTACCCTGTCCCTTTTCCTACAATGATTTTT NGG 10 8 17 8 24 0 100 5 10
    240606172530_j6hj CATAGAAAAATCATTGTAGGAAAAGGGACAGG(+AAAAAA)GTACCAAGGAGGATCTGGGCACATCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606172528_a4we AATGAGTATCATAGATGGTCAGGCTGCAGATG(+AAAAAA)GATCCCAGAACCTGCAGCTACCACGGACACC NGG 10 8 17 8 24 0 100 5 10
    240606172527_842j CTCCTGGTGTCCGTGGTAGCTGCAGGTTCTGG(+AAAAAA)GATCCATCTGCAGCCTGACCATCTATGATAC NGG 10 8 17 8 24 0 100 5 10
    240606172525_vszw AATGACCCTCACTTAGGAAGCAGTCATGATAG(+AAAAAA)GTACCCAAACTTTTAAACCAGGCAGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606172524_k868 TCACTGTGCTCCTGCCTGGTTTAAAAGTTTGG(+AAAAAA)GTACCTATCATGACTGCTTCCTAAGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606172522_9xc8 GCAGACATCTCTACCTCTTAACCCAGAGTTCG(+AAAAAA)GATCCCTCGGACTTCCAAAATCACACCTCTG NGG 10 8 17 8 24 0 100 5 10