Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174259_pn6e GAAGTGTCCATGAAGACTGCAACACAGCATTG(+AAAAAA)GATCCCCCTGAGCTGCAGTTACAGACATTTG NGG 10 8 17 8 24 0 100 5 10
    240606174257_4nz5 GCTCACAAATGTCTGTAACTGCAGCTCAGGGG(+AAAAAA)GATCCAATGCTGTGTTGCAGTCTTCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606174255_03bc CCATAAAGTCTCTGACCATCTCTGGAATCAGG(+AAAAAA)GATCCACCCAGACAGCCTAGACATAGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240606174253_giox GTCAGATGCTCTATGTCTAGGCTGTCTGGGTG(+AAAAAA)GATCCCTGATTCCAGAGATGGTCAGAGACTT NGG 10 8 17 8 24 0 100 5 10
    240606174252_e03g AGCTACATCCTAAGTTCTGAGTGAGGGGGTAG(+AAAAAA)GATCCACAGGAAGAAATAAGTCATGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606174250_bus3 CTCCCCAAGCTCATGACTTATTTCTTCCTGTG(+AAAAAA)GATCCTACCCCCTCACTCAGAACTTAGGATG NGG 10 8 17 8 24 0 100 5 10
    240606174249_nk5y CCCAGCCCAGCCGCCACCAGCCTCTCTGATTG(+AAAAAA)GATCCCCTTAACTGCATGCTCTTGAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240606174247_62w5 CCCCATGGGGTTCAAGAGCATGCAGTTAAGGG(+AAAAAA)GATCCAATCAGAGAGGCTGGTGGCGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606174246_c0er GGCAAGTCTGCCTAGCTGTGCTCTGAGGACAG(+AAAAAA)GATCCTAACTGACAGCCTACACTTTGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606174244_2i9f TGTTAATCTACAAAGTGTAGGCTGTCAGTTAG(+AAAAAA)GATCCTGTCCTCAGAGCACAGCTAGGCAGAC NGG 10 8 17 8 24 0 100 5 10