Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172323_odi7 GCTAGGAACTCACGTCACAGGGTTGTTGTGAG(+AAAAAA)GATCCAGTGAGCTGATTGGGTAGAGCACCTA NGG 10 8 17 8 24 0 100 5 10
    240606172322_51ip CACTAAAACTGGCTCACCTCATCTAGGTTAGG(+AAAAAA)GTACCTATCGTTGAGTTTATTAGACAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606172320_yelu CAACTCTAACTGTCTAATAAACTCAACGATAG(+AAAAAA)GTACCCTAACCTAGATGAGGTGAGCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606172318_3woi TGCTAGAGTTATTGTTACAATAATACAGGCCG(+AAAAAA)GTACCTACTGTACAGAGTTAAAACTATATGG NGG 10 8 17 8 24 0 100 5 10
    240606172317_apip TAAAGCCATATAGTTTTAACTCTGTACAGTAG(+AAAAAA)GTACCGGCCTGTATTATTGTAACAATAACTC NGG 10 8 17 8 24 0 100 5 10
    240606172314_uiek CCCCTTAACACCATCTCCAGCCTCATCCTAGG(+AAAAAA)GTACCAGCTCAAAACAATACCGGGAGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240606172313_g90a CCGCCTGCTTCTCCCGGTATTGTTTTGAGCTG(+AAAAAA)GTACCCTAGGATGAGGCTGGAGATGGTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606172311_und7 AGCAAGCTCGGGAGCTCCCAGAATCTGCTTAG(+AAAAAA)GATCCACTCCAGGGCTTGGAGAGGCGATGAA NGG 10 8 17 8 24 0 100 5 10
    240606172310_wffg GTTTGTTCATCGCCTCTCCAAGCCCTGGAGTG(+AAAAAA)GATCCTAAGCAGATTCTGGGAGCTCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240606172308_ws7u AGACCTGGATCTCACTTGGATAAGAGTTCTGG(+AAAAAA)GATCCTGCCATGATCCTTGAGAAAGAAAAAT NGG 10 8 17 8 24 0 100 5 10