Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172220_tnh9 ACAGCCAGAGCAGGGTTGTGTGTTGTTTTGGG(+AAAAAA)GTACCACACTAGCCCCTCTTTCGAACTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606172218_3p0m CTATTCTCCTTCCAGCCAGCCAGAAATCCTCG(+AAAAAA)GTACCTGCACCAGTGACCCAGCAGTCGCACA NGG 10 8 17 8 24 0 100 5 10
    240606172217_017z CTCACTGTGCGACTGCTGGGTCACTGGTGCAG(+AAAAAA)GTACCGAGGATTTCTGGCTGGCTGGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606172215_n5do AACATTCCAGTTTCCCACAGTCTTATCTTTGG(+AAAAAA)GTACCGTTTACAAACTCATGAGGTTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240606172214_yj5r TTACAGAGGTCAACCTCATGAGTTTGTAAACG(+AAAAAA)GTACCCAAAGATAAGACTGTGGGAAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240606172212_lqvd CAGAAAGATGGAAACGATCAGGGACAGCCCAG(+AAAAAA)GATCCCTGATGAGAACTCATGATTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240606172210_79z5 AATGGCGCAAAAAATCATGAGTTCTCATCAGG(+AAAAAA)GATCCTGGGCTGTCCCTGATCGTTTCCATCT NGG 10 8 17 8 24 0 100 5 10
    240606172209_ziwz CCCTAGTTTCTGCCCTCCACACATCATCAGTG(+AAAAAA)GTACCTTAAGTCTTATTCCTGCCAAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606172207_087z GATCTTTCCTTTTGGCAGGAATAAGACTTAAG(+AAAAAA)GTACCACTGATGATGTGTGGAGGGCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606172206_iwvl CTGTTGCCTGTGAAGGTCAGAAAAGGGCAGTG(+AAAAAA)GATCCCATGAGGCTGGATTTATAGATGGTTG NGG 10 8 17 8 24 0 100 5 10