Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172134_snry TGGGCTGGTGTGTAGCTCAGGACCCTTGTTTG(+AAAAAA)GATCCCCAGGGTTACTAAGAAGAAAAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606172132_gm7q TAGAAGTCTTCAGAGGTCAGAAGACAGCACTG(+AAAAAA)GATCCCCTGGAGCTGGAGTTATGGATGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606172130_pfmy GGCCACCACCATCCATAACTCCAGCTCCAGGG(+AAAAAA)GATCCAGTGCTGTCTTCTGACCTCTGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606172128_3ep3 AGATAGAAGCTCAACTTGACAAGATTCGTCTG(+AAAAAA)GATCCCAGGGTAATGCAAGACAAACCCACAC NGG 10 8 17 8 24 0 100 5 10
    240606172126_v02e AAATGGTGTGGGTTTGTCTTGCATTACCCTGG(+AAAAAA)GATCCAGACGAATCTTGTCAAGTTGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606172125_5wyg CAGTGGCGAGGGATGGTCTCTAGTCTTCTCTG(+AAAAAA)GTACCTCAGCATCCCCAAAGGCTCTTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606172124_1epz TGGAAGCCAAAAGAGCCTTTGGGGATGCTGAG(+AAAAAA)GTACCAGAGAAGACTAGAGACCATCCCTCGC NGG 10 8 17 8 24 0 100 5 10
    240606172122_bcsk AGTTGAGTCAGGTGGCAGACAGTTTCCACCAG(+AAAAAA)GATCCAAGCCTCTGCGTATGCAGGGATATAT NGG 10 8 17 8 24 0 100 5 10
    240606172120_vlq0 AAGCCATATATCCCTGCATACGCAGAGGCTTG(+AAAAAA)GATCCTGGTGGAAACTGTCTGCCACCTGACT NGG 10 8 17 8 24 0 100 5 10
    240606172118_oskj GGCCTTGGCTCTCACAGTTCGAGAGAACCAGG(+AAAAAA)GATCCTCTCGAAAGATGCAACTGCCCAACAG NGG 10 8 17 8 24 0 100 5 10