Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172041_g3ek AAGAGGGTGCTCCGAGCTAGGGCGGCAGCACG(+AAAAAA)GATCCCGGCTGCTCAGTTTGTGTCGCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606172040_v6z1 ACACGACGCTGCCTCTGCCTCTCCAGCACTGG(+AAAAAA)GATCCCAAGCCAGGCCTTTCATGTGAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606172038_7v3x TCCCCAGCACTCACATGAAAGGCCTGGCTTGG(+AAAAAA)GATCCCAGTGCTGGAGAGGCAGAGGCAGCGT NGG 10 8 17 8 24 0 100 5 10
    240606172036_bsh4 TGCTCCACCCACACAGGTGCAGCAATATGGGG(+AAAAAA)GTACCACCTCAAATGCTTTGTCATGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606172035_yn0v AAGGATCTCTGCATGACAAAGCATTTGAGGTG(+AAAAAA)GTACCCCCATATTGCTGCACCTGTGTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606172033_0rvi TCAAACCACTGAGCTACTACACAATGTGGAGG(+AAAAAA)GATCCATTTAAATCAATAAAACGACAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606172032_tf4n AACAATGGGCTGTCGTTTTATTGATTTAAATG(+AAAAAA)GATCCCTCCACATTGTGTAGTAGCTCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606172030_spab AGGCTGGATGTGCACAGCTAGTTGGGCGGATG(+AAAAAA)GTACCAAGTCCTATGGAAACTGTTCTATTCC NGG 10 8 17 8 24 0 100 5 10
    240606172029_4007 CCAAAGGAATAGAACAGTTTCCATAGGACTTG(+AAAAAA)GTACCATCCGCCCAACTAGCTGTGCACATCC NGG 10 8 17 8 24 0 100 5 10
    240606172027_uhk7 GGGTGGGCTGACCGTAAACTGACCAGCGCTGG(+AAAAAA)GATCCCACACTTGTTTTATACTCATCTCTAG NGG 10 8 17 8 24 0 100 5 10