Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172026_zgun GTAACCTAGAGATGAGTATAAAACAAGTGTGG(+AAAAAA)GATCCCAGCGCTGGTCAGTTTACGGTCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606172024_ppek TAATGTGTCATGTGTTATATTAATCTCTATTG(+AAAAAA)GTACCTGACATTGTTTCTTTGAGCAGTATGA NGG 10 8 17 8 24 0 100 5 10
    240606172023_kj9w TTGCATCATACTGCTCAAAGAAACAATGTCAG(+AAAAAA)GTACCAATAGAGATTAATATAACACATGACA NGG 10 8 17 8 24 0 100 5 10
    240606172022_p7th CTTATGACTTAGAATAATATTTTTTTCACATG(+AAAAAA)GATCCACCAGGGTTAGTTTCAGAGTCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240606172020_rdfz TTCCAGTACTGACTCTGAAACTAACCCTGGTG(+AAAAAA)GATCCATGTGAAAAAAATATTATTCTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606172018_nxul AGGCATGCTGTGTCTGAAGTGTTTAATAAAGG(+AAAAAA)GATCCTTGTTACAGAACTTGTCTTCCGATGC NGG 10 8 17 8 24 0 100 5 10
    240606172017_6lct GGTGTGCATCGGAAGACAAGTTCTGTAACAAG(+AAAAAA)GATCCCTTTATTAAACACTTCAGACACAGCA NGG 10 8 17 8 24 0 100 5 10
    240606172005_x1ig GTGTTTGTGAAGCCCGAACAGTGTGAGTCCGG(+AAAAAA)GTACCGTGTGTACATACATATGTAGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606172004_qm5m AAACGTTATTCCTACATATGTATGTACACACG(+AAAAAA)GTACCCGGACTCACACTGTTCGGGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240606172002_iz4s ACCCAATATCTCCTACACCTAGTTGCAATAGG(+AAAAAA)GTACCTGTGCCCTGGACTTTCTCCTTACATG NGG 10 8 17 8 24 0 100 5 10