Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171914_zjcj ATCTGCTCGCCTCCCTCTAGACTGGCCCACTG(+AAAAAA)GATCCATACATTTGCTTATCTGCTCCGATCA NGG 10 8 17 8 24 0 100 5 10
    240606171912_9yda GCCTGTCATGGTATACCCCTGCTAGCCTTGGG(+AAAAAA)GATCCATAAGGAGACCCCTAGCATGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606171910_hrqw AAGTACAGGGCCATGCTAGGGGTCTCCTTATG(+AAAAAA)GATCCCCAAGGCTAGCAGGGGTATACCATGA NGG 10 8 17 8 24 0 100 5 10
    240606171909_4blo TGGCACTGTTACCACTTTTGAAAGGTTGTTGG(+AAAAAA)GTACCAGGGTAATCCCACTAAATGTGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606171906_pyss AAGTATACTCCACATTTAGTGGGATTACCCTG(+AAAAAA)GTACCCAACAACCTTTCAAAAGTGGTAACAG NGG 10 8 17 8 24 0 100 5 10
    240606171904_0cq7 TGCTGTGAACTAGAACCGTGGGTAGAGGCCGG(+AAAAAA)GTACCCTGGGGTTTGTGGAGAAGGTGTCACT NGG 10 8 17 8 24 0 100 5 10
    240606171901_zrq1 GGCACAGTGACACCTTCTCCACAAACCCCAGG(+AAAAAA)GTACCCGGCCTCTACCCACGGTTCTAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606171859_evyh CAGCCCCCATCAAGTCCCTGTGCAAACTGGAG(+AAAAAA)GTACCAGCTGGCTTCAGGATGGGGCACCATA NGG 10 8 17 8 24 0 100 5 10
    240606171858_t416 GGAGATATGGTGCCCCATCCTGAAGCCAGCTG(+AAAAAA)GTACCTCCAGTTTGCACAGGGACTTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240606171856_qiao CACTTGTGCTGTTGGCTCATATTTTCACCTTG(+AAAAAA)GATCCTCTTACCTGTTGCCCAAGCCTCTGAG NGG 10 8 17 8 24 0 100 5 10