Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171929_qr0j CTGGACAACCTGGCCTTCCCTGTGCCCTCCTG(+AAAAAA)GATCCCAGGATGTGTAGAGGTCTGCAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240606171928_y06d TACTTGTCCTAGCATTCGGAAGAGGAAAGCAG(+AAAAAA)GATCCCAGCAAATAAACCAGCCAGCCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606171926_fw9i TTCTTTGTTTGGCTGGCTGGTTTATTTGCTGG(+AAAAAA)GATCCTGCTTTCCTCTTCCGAATGCTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606171925_ekcb CGCACACAGGTGACAGAGAGCTGGGGCTTCTG(+AAAAAA)GTACCTTAGCTTGGATTCAGACACTCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240606171923_aphp TGCTTTACCTGAGTGTCTGAATCCAAGCTAAG(+AAAAAA)GTACCAGAAGCCCCAGCTCTCTGTCACCTGT NGG 10 8 17 8 24 0 100 5 10
    240606171921_0zes CACTGGGAACTGGGATTACAGATGTGAGCAGG(+AAAAAA)GATCCAGCTTGTTACAGACACACCTGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606171920_akmn TAGTCTCAGCCAGGTGTGTCTGTAACAAGCTG(+AAAAAA)GATCCCTGCTCACATCTGTAATCCCAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606171918_gbn7 CTTCGAACCAAGGCTCTACAAAGCACCTCAGG(+AAAAAA)GTACCTTTCACAGCCCAGAACTCTAGACACA NGG 10 8 17 8 24 0 100 5 10
    240606171917_z4b4 CTTCCTGTGTCTAGAGTTCTGGGCTGTGAAAG(+AAAAAA)GTACCCTGAGGTGCTTTGTAGAGCCTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606171916_q9qw TCAATTGATCGGAGCAGATAAGCAAATGTATG(+AAAAAA)GATCCAGTGGGCCAGTCTAGAGGGAGGCGAG NGG 10 8 17 8 24 0 100 5 10