Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172356_nbjm GAAGTTCTGCATCTATTCCCAGGAGCCCAGTG(+AAAAAA)GATCCACAGCTGCCCAGGTCCTTAAGCAATC NGG 10 8 17 8 24 0 100 5 10
    240606172355_actx TAGTGAAGACAACTCTGACCTTGGCATAAAAG(+AAAAAA)GATCCAAGTCAGAATCCCAGCTTGGTCAATT NGG 10 8 17 8 24 0 100 5 10
    240606172354_2esu GTATTAATTGACCAAGCTGGGATTCTGACTTG(+AAAAAA)GATCCTTTTATGCCAAGGTCAGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606172353_xnea TAGAGCTGCCCACATCACTGATCCTGCTTATG(+AAAAAA)GATCCTCTCCTCTCTGTGGATGTACCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606172351_4uap CAGGACACAGGGTACATCCACAGAGAGGAGAG(+AAAAAA)GATCCATAAGCAGGATCAGTGATGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606172350_k4lw TAAAAGGAAAAGGGAAGCCACTGCTACTGGAG(+AAAAAA)GATCCCCTTCAGTTTTACAAAAAGCTCATCT NGG 10 8 17 8 24 0 100 5 10
    240606172348_31p7 GTTCAAGATGAGCTTTTTGTAAAACTGAAGGG(+AAAAAA)GATCCTCCAGTAGCAGTGGCTTCCCTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606172347_9ffp GTGCATCTTACAACAGCTAAGCATTTATTAAG(+AAAAAA)GATCCTCATGAGATACAAGATTAGGGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606172345_igd5 CCACATGTCTCCCTAATCTTGTATCTCATGAG(+AAAAAA)GATCCTTAATAAATGCTTAGCTGTTGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606172343_ytwx ATGGTGGCACTAATTTCTGAAATTCATCCAGG(+AAAAAA)GATCCAAATACTGTTTTTGAGTTCAGTTTTC NGG 10 8 17 8 24 0 100 5 10