Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172450_x0vj CAAGTTTTAATTTAGAATTATCAGATTCATTG(+AAAAAA)GTACCAGGATATCCTGTAGTGTTGCTAACTG NGG 10 8 17 8 24 0 100 5 10
    240606172448_ev8i CTCACCCCTTACTGAGAATCTATTCACAATTG(+AAAAAA)GTACCTATTGGGAAAGGGAAAACCAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606172447_zn9c TTAGAAAAAGCTGGTTTTCCCTTTCCCAATAG(+AAAAAA)GTACCAATTGTGAATAGATTCTCAGTAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606172446_vqvz ATGAGATCTTTTTTTACCCCTTTAACATTCAG(+AAAAAA)GTACCCTAGTAGAGAAAAATAAATAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606172444_x8my TTTTATTTATTTATTTATTTTTCTCTACTAGG(+AAAAAA)GTACCTGAATGTTAAAGGGGTAAAAAAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606172442_rm73 TGAGACCGCCATCCATTCCGTTCCAGGAATGG(+AAAAAA)GTACCATGGCTTCTGGGAAGCAACATCCACG NGG 10 8 17 8 24 0 100 5 10
    240606172429_9s1f ATCCCCGTGGATGTTGCTTCCCAGAAGCCATG(+AAAAAA)GTACCCATTCCTGGAACGGAATGGATGGCGG NGG 10 8 17 8 24 0 100 5 10
    240606172428_vpco TATTTACCTACTTATTAAGGGAGATAATTTTG(+AAAAAA)GTACCCTTGGTTATCAGGTAGTAGAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606172426_ifsq ATTCTCCCACCTCTACTACCTGATAACCAAGG(+AAAAAA)GTACCAAAATTATCTCCCTTAATAAGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606172424_ttu9 ATTCCCCAGAGGAACATTGAGCAGAGTAAATG(+AAAAAA)GTACCCGTCACTGATTGCAAGCCAATGTATC NGG 10 8 17 8 24 0 100 5 10